Designed method validation for new In-Vitro Dissolution by UV Visible Spectrophotometer for Metronidazole extended releases tablet by single chemical.

Abstract

The present research work discusses the development of a UV spectrophotometric method for Metronidazole. Simple, accurate and cost efficient spectrophotometric method has been developed for the estimation of Metronidazole (MND) in Tablet dosage form. The optimal conditions for the drug analysis were established. The maximum wavelength (max) was found to be 278nm. The percentage recovery of the drug was calculated at 8 hours and obtained 99.4 % against criteria -Recovery should not be less than 95.0 and linearity observed as 0.9999. Validation was conducted in accordance with ICH guidelines, covering parameters such as linearity, accuracy, precision, limit of detection (LOD), and limit of quantification (LOQ). The sample solution exhibited stability for up to 24 hours. The proposed method is deemed appropriate for the analysis of Metronidazole in tablet formulations for quality control applications.