VALIDATED HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF ZINC CARNOSINE AND ACECLOFENAC IN BULK AND TABLET DOSAGE FORM

Authors

  • Panjagala Satyanarayana School of Pharmaceutical Sciences, Vels University, Zameen Pallavaram, Chennai, India – 600 117.
  • Shanmugasundaram P School of Pharmaceutical Sciences, Vels University, Zameen Pallavaram, Chennai, India – 600 117.
  • Sudarshan reddy P School of Pharmaceutical Sciences, Vels University, Zameen Pallavaram, Chennai, India – 600 117.
  • Chandra mohan rao G School of Pharmaceutical Sciences, Vels University, Zameen Pallavaram, Chennai, India – 600 117.

Keywords:

HPLC, Zinc carnosine & Aceclofenac method development, validation, Tablet.

Abstract

A validated HPLC method for simultaneous estimation of Zinc carnosine and Aceclofenac in pharmaceutical dosage forms. Chromatography was carried out on a C18 column [250mm, 4.6m, 5µm] using a mixture of potassium di-hydrogen phosphate  buffer: Acetonitrile: methanol (50:30:20  v/v) as  the  mobile  phase  at  a flow  rate  of  1 ml/min.  Detection was carried out by using PDA detector. The retention time of the drug was 2.258 and 6.690 min. The method produced linear responses in the concentration range of 2 to 10μg/ml for both drugs. The LOD and LOQ values were found to be 33.4,

101.3 and 2.473, 7.495 ng/ml respectively. The  method was validated for linearity, precision, accuracy, LOD &LOQ in accordance with ICH guidelines. The proposed method was found to be applicable for determination of the drug in tablet dosage forms.

Dimensions

Published

2011-05-11

Issue

Vol. 1 No. 2 (2011): 2011 Volume -1 - Issue 2

Section

Articles
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