Simultaneous estimation of new analytical rp-hplc method development and validation of propranolol and clonazepam

Authors

  • Sanaboina Durga Sowjanya Department of Pharmaceutical Quality Assurance, Pydah College of Pharmacy Patavala, Andhra University, Kakinada, Andhra Pradesh, India.
  • A. Venkateswara Rao Department of Pharmaceutical Quality Assurance, Pydah College of Pharmacy Patavala, Andhra University, Kakinada, Andhra Pradesh, India.
  • D. Appalaraju Department of Pharmaceutical Quality Assurance, Pydah College of Pharmacy Patavala, Andhra University, Kakinada, Andhra Pradesh, India.

Keywords:

Clonazepam, Propranolol, RP-HPLC, validation

Abstract

A rapid and precise Reverse Phase High Performance Liquid Chromatographic method has been developed for the validated of Clonazepam and Propranolol, in its pure form as well as in tablet dosage form. Chromatography was carried out on a Zorbax C18 (4.6 x 150mm, 5µm) column using a mixture of Methanol: Water (70:30) as the mobile phase at a flow rate of 1.0ml/min, the detection was carried out at 265 nm. The retention time of the Clonazepam and Propranolol was 2.061, 2.462 ±0.02min respectively. The method produce linear responses in the concentration range of 3-15µg/ml of Clonazepam and 60-300µg/ml of Propranolol. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the quality control of bulk and pharmaceutical formulations.

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Published

2023-09-11

Issue

Vol. 13 No. 3 (2): 2023 Volume -13 - Issue 3

Section

Articles
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