Validation of simple isocratic RP-HPLC method for clonazepam and propranolol HCL determination and it’s application in the study of stress degradation

Abstract

A easy, exact, precise technique was developed for the synchronous estimation of the clonazepam and propranolol

hcl in pharmaceutical measurement frame. Chromatographic conditions utilized are standard BDS C8 Column(150 x

4.6mm, 5m) utilizing a mobile phase comtain of buffer 0.01N KH2PO4 (4.8pH): Acetonitrile taken in the ratio of

60:40 v/v at a flow rate of 1ml/min with UV recognition at238nm. Buffer utilized in this technique was 0.01N

KH2PO4. Temperature was kept up at 30oC. Retention time of propranolol hcl and clonazepam were observed to be

2.267min and 2.983min correspondingly. %RSD of the propranolol hcl and clonazepam were observed to be 0.5 and

0.correspondingly. %Recovery was get as 100.08% and 100.64% for propranolol hcl and clonazepam respectively.

LOD, LOQ values get regression equations of propranolol hcl and clonazepam were 0.04, 0.11 and 0.02, 0.07

correspondingly. Regression equation of propranolol hcl is y = 39145x + 5375, and y = 79887x + 1421 of

clonazepam. The developed method was approved in terms of precision, linearity, exactness, LOD, LOQ and

specificity are in acknowledgment criteria, so the technique developed was easy and economical that can be adopted

in regular Quality control tests in ventures.