Analytical Method Development And Validation For Simultaneous Estimation Of Ranitidine And Ondansetron In Pure And Its Tablet Dosage Form By Rp-Hplc

Authors

  • Gubba Mythile Department Of Pharmaceutical Analysis, Holy Mary Institute Of Technology And Science (College Of Pharmacy), Keesara - Bogaram - Ghatkesar Rd, Kondapur, Telangana 501301
  • D. Venkata Ramana Department of Pharmaceutics, Holy Mary Institute of Technology & Science (College of Pharmacy), Bogaram Village, Keesara Mandal, Hyderabad, Telangana, India
  • Udaya Bhanu Sri Koppu Department of Pharmaceutics, Holy Mary Institute of Technology & Science (College of Pharmacy), Bogaram Village, Keesara Mandal, Hyderabad, Telangana, India
  • K. Sai Kiran Department of Pharmaceutics, Holy Mary Institute of Technology & Science (College of Pharmacy), Bogaram Village, Keesara Mandal, Hyderabad, Telangana, India

Keywords:

Ondansetron, Ranitidine, RP-HPLC, validation.

Abstract

A rapid and precise reverse phase high performance liquid chromatographic method has been developed for the validated of Ranitidine and Ondansetron, in its pure form as well as in tablet dosage form. Chromatography was carried out on a Hypersil C18 (4.6×250mm) 5µ column using a mixture of Water and Acetonitrile (50:50) as the mobile phase at a flow rate of 1.0ml/min, the detection was carried out at 244nm. The retention time of the Ondansetron and Ranitidine was 2.0, 4.0±0.02min respectively. The method produce linear responses in the concentration range of 5-25µg/ml of Ondansetron and 93.75-468.75µg/ml of Ranitidine. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the quality control of bulk and pharmaceutical formulations.

Dimensions

Published

2024-02-14

Issue

Vol. 14 No. 1 (2024): 2024 Volume -14 - Issue 1

Section

Articles
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