SIMULTANEOUS ESTIMATION OF VALSARTAN AND HYDROCHLOROTHIAZIDE IN TABLETS BY RP-HPLC METHOD

Abstract

A simple, reproducible and efficient reverse phase high performance liquid chromatographic method was developed for simultaneous determination of valsartan and hydrochlorothiazide in tablets. A column having 100mmx4.6mm i.d. in isocratic mode with mobile phase containing Acetonitrile: Phosphate buffer (50:50; adjusted to pH 3.0) was used. The flow rate was 0.8 ml/min and effluent was monitored at 225 nm. The retention time (min) and linearity range (μg/ml) for valsartan and hydrochlorothiazide were (5.59, 2.36) and (10-50, 10-50), respectively. The developed method was found to be accurate, precise and selective for simultaneous determination of valsartan and hydrochlorothiazide in tablets.