EFFECT OF PLANT GROWTH REGULATORS ON IN VITRO PROPAGATION OF RHINACANTHUS NASUTUS L. (ACANTHACEAE)

Abstract

The purpose of this study was to develop a new micropropagation system for Rhinacanthus nasutus” (Acanthaceae) an important medical plant in India. In this present study a standard protocol for micro propagation has been raised by the use of various explants inoculated on MS media fortified with different concentrations and combinations of cytokinin (BAP). The maximum number of 2.8 ± 1.2 shoots were proliferated per explants on MS media supplemented with BAP (0.1, 0.5, 1, 1.5, 2.0 mg/l). Multiple shoots were observed on media supplemented with 2.0 mg/l from the explants. The nodal explants were inoculated on MS media fortified with different concentrations and combinations of Auxin (IBA). Maximum numbers of roots were proliferated per explant, supplemented with IBA 1.5mg/l. White nodular callus pronounced from the leaf explants supplemented, when they were subjected to 2,4-D at different concentrations.