MICROSCOPICAL OBSERVATION AND BIOCHEMICAL ANALYSIS OF LIVER FOR HEPATOPROTECTIVE EFFECT OF VITAMIN - E & C IN ALBINO RATS

Abstract

The Hepatoprotective effects of Vitamins E and C (VEC) were evaluated in Carbon tetrachloride (CCl₄) or Paracetamol (PC) induced hepatotoxicity in albino rats. Liver necrosis was induced by administering single dose of either carbon tetrachloride (CCl₄, 1ml/k g, 50% v/v with olive oil, s.c.) or Paracetamol (PC, 1g/kg, p.o.). The liver damage was evidenced by Microscopic observation of Hepatic lobule configuration and the analysis of the levels of Serum glutamate oxaloacetate transaminase (SGOT), Serum glutamate pyruvate transaminase (SGPT), Serum alkaline phosphatase (ALP), Hepatic Thio-barbituric acid reacting substances (TBARS) and Superoxide dismutase (SOD) . VEC treatment (50/100 mg/kg Vitamin-E and 100/200 mg/kg Vitamin-C) by intraperitoneal injection, significantly (P<0.001) reduced CCl₄ or PC- induced elevations of the levels   of SGOT, SGPT, ALP and SOD, while it reduced the concentration of TBARS. Microscopical analysis using Hematoxylin and Eosin (H&E) stain of the VEC administered rats’ revealed remarkable normal Hepatic lobule configuration, unlike the hepatotoxic rats whose hepatic cells were necrotic.