Method development and validation for simultaneous estimation of fluorescein and benoxinate by RP-HPLC

Abstract

A short selective, precise, accurate and sensitive method for the estimation of benoxinate and fluorescein was

done by RP-HPLC. The chromatographic separation was clear at the flow rate of 1 ml/min, at UV detection of

358 nm. The assay for benoxinate and fluorescein were found to be 101.9 and 99.7 respectively. which shows

that the method is useful for routine analysis. The linearity of benoxinate and fluoresceinwas found to be direct

with a relationship coefficient of 0.9968 and 0.9962, which appears that the strategy is competent of creating

great affectability. The vigor restrain for versatile stage variety and stream rate variety are well inside the

constrain, the % debasement comes about are in limits. Which appears that the strategy is having great

framework reasonableness and accuracy beneath given set of conditions. From the recovery and the studies,

showing acceptable limits, it can be concluded that this can be employed for estimation of benoxinate and

fluorescein in its dosage forms