Determination of Gallic acid content from marketed monoherbal formulations containing Emblica officinalis by HPLC technique

Abstract

Standardization refers to the process of delivering a product with a specified minimum level of one or more plant constituents. In some cases this is accomplished by measuring the level of a chemical in a crude herbal extract and establishing a standard amount of that chemical for future production. The standardization of herbal formulations has become very essential as there is increase in the demand and frequent usage of the herbal formulations by the people. Monoherbal formulations containing Emblica officinalis extract were collected from the commercial market and standardized for their gallic acid content by High Performance Liquid Chromatographic Technique (HPLC). The Tablet and Capsule forms of different brands of marketed herbal formulations of Amla were selected and analysed by HPLC Technique. The total peak area of standard (gallic acid) and the corresponding peak area of samples were compared and the amount present in it was calculated. The results reveal that there are lot of variations between the
samples and the percentage of gallic acid is not uniform. The present study indicates the necessity of development of
analytical procedures for all herbal formulations available in the market to ensure the quality and efficacy of the
products