Estimation of Ascorbic Acid from Emblica Officinalis collected from different Geographical Sources by HPLC Technique

S.Manimaran; M.Ranjith Raj; S.Zahir Hussain; R.Ranjithkumar; R.Aravindan; R.Ajith; G.Arunachalam; R. Joseph Sahaya Raja; R.Prasath

Authors

S.Manimaran
editorijpir@gmail.com
Department of Pharmacognosy, P.G.P.College of Pharmaceutical Science and Research Institute, Namakkal, Tamilnadu, India.
M.Ranjith Raj Department of Pharmacognosy, P.G.P.College of Pharmaceutical Science and Research Institute, Namakkal, Tamilnadu, India.
S.Zahir Hussain Department of Pharmacognosy, P.G.P.College of Pharmaceutical Science and Research Institute, Namakkal, Tamilnadu, India.
R.Ranjithkumar Department of Pharmacognosy, P.G.P.College of Pharmaceutical Science and Research Institute, Namakkal, Tamilnadu, India.
R.Aravindan Department of Pharmacognosy, P.G.P.College of Pharmaceutical Science and Research Institute, Namakkal, Tamilnadu, India
R.Ajith Department of Pharmacognosy, P.G.P.College of Pharmaceutical Science and Research Institute, Namakkal, Tamilnadu, India.
G.Arunachalam Department of Pharmacognosy, P.G.P.College of Pharmaceutical Science and Research Institute, Namakkal, Tamilnadu, India.
R. Joseph Sahaya Raja Synthiya Research Labs Pvt. Ltd., Pondicherry.
R.Prasath Synthiya Research Labs Pvt. Ltd., Pondicherry.

Keywords:

HPLC Analysis, Emblica officinalis, Raw material, Ascorbic acid

Abstract

Standardization of herbal drug or extract means confirmation of its identity, determination of its quality and purity by carry out the proper approved procedures. In order to obtain quality oriented herbal products, care should be taken right from the proper identification of plants, season and area of collection and their extraction and purification process. The standardization of herbal raw materials has become very important as there is increase in demand. The herbal raw materials containing Emblica officinalis fruits were collected from various geographical sources and standardized for their ascorbic acid content by High Performance Liquid Chromatographic Technique (HPLC) is our objective of present study. The collected fresh fruits were cut into small pieces, dried under shade and made to fine powder. The powdered raw materials were subjected to HPLC analysis to estimate the ascorbic acid content. The percentage of ascorbic acid was estimated by comparing the peak area of standard and the same present in the sample. The results reveal that there are some variations between the samples and the percentage of ascorbic acid is not uniform in all the collected samples. The content of ascorbic acid is not uniform in all the collected samples and it is concluded that the variation may be due the soil & soil fertility and climatic conditions.

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