Stability indicating RP-HPLC method development and validation for  estimation of ivabradine and metoprolol in pharmaceutical dosage form

Authors

K. Krishna Rayulu
editorijipsr@gmail.com
Department of Pharmaceutical Chemistry, Malla Reddy College of Pharmacy, Osmania University, Maisammaguda, Hyderabad, India.
Sridhar Siddiraju Department of Pharmaceutical Chemistry, Malla Reddy College of Pharmacy, Osmania University, Maisammaguda, Hyderabad, India.
S.B. Bhanja Department of Pharmaceutical Chemistry, Malla Reddy College of Pharmacy, Osmania University, Maisammaguda, Hyderabad, India.

Ivabradine, Metoprolol, RP-HPLC

A simple, Accurate, precise method was developed for the simultaneous estimation of the Ivabradine and

Metoprolol in tablet dosage form. Chromatogram was run through Ascentis C18 150mm x 4.6 mm, 3.6m.

Mobile phase containing 0.01N KH2PO4: Acetonitrile taken in the ratio 60:40 was pumped through column at a

flow rate of 0.8 ml/min. Temperature was maintained at 30°C. Optimized wavelength selected was 260.0nm.

Retention time of Ivabradine and Metoprolol were found to be 2.181min and 2.669min. %RSD of the

Ivabradine and Metoprolol were and found to be 0.6 and 0.3 respectively. %Recovery was obtained as

100.37% and 99.54% for Ivabradine and Metoprolol respectively. LOD, LOQ values obtained from regression

equations of Ivabradine and Metoprolol were 0.11, 0.33 and 0.39, 1.19 respectively. Regression equation of

Ivabradine is y = 5200.x + 310.0 and y = 9807.x + 6086 of Metoprolol. The method developed was simple and

economical that can be adopted in regular Quality control test in Industries.

Dimensions