Design characterization of atomoxetine loaded solid lipid nanoparticle

Abstract

Nano-materials with excellent biodegradability and biocompatibility are considered to be the best vehicles for drug delivery systems in biomedical applications.  Currently, scientists and researchers are focused on discovering new methods/routes to control the pharmacokinetics (ADME), pharmacodynamics, non-specific toxicity, immunogenicity, biorecognition, and drug efficacy of drugs.  These new strategies are often called novel drug delivery systems (NDDS) and are based on interdisciplinary approaches that combine polymer science, pharmaceutics, bioconjugate chemistry, and molecular biology.

To formulate and characterize the Atomoxetine loaded Solid lipid nanoparticle by solvent evaporation method. To formulate the solid lipid nanoparticles of Atomoxetine. To study the drug-polymer compatibility by FTIR and to characterize the solid lipid nanoparticles for particle size, Entrapment Efficiency, zetapotential. To study the drug release of SLN formulations and the drug release kinetics of SLN, and stability of optimized SLN formulation.

The In-vitro drug release for all nine formulations was evaluated by the dialysis bag diffusion technique. In-vitro drug release for the optimized FSLNA7. Showed the best result by releasing atomoxetine 99.01% in 12hr. The drug release kinetics was calculated by fitting the dissolution data in various equation the results in Table No.9 depicting the best formulation is following zero order kinetics results.

In the present research work the solid lipid nanoparticle of Atomoxetine was formulated by solvent evaporation method, The ingredient s like lipid (stearic acid), surfactant (tween 20) and co-surfactant (PG) were selected for formulation using methanol as solvent. A solution of Atomoxetine in phosphate buffer pH 7.4 was scanned in UV range between 200 to 350 nm (LabIndiaUV 3200 spectrophotometer, India). Atomoxetine showed maximum absorbance at 274 nm in phosphate buffer pH7.4. FT-IR studies suggested that there is no interaction between the drug and the polymer. It was concluded that the Atomoxetine loaded Solid lipid nanoparticle FSLNA7 is the best formulation can be considered for animal study.