Formulation and evaluation bio-silver nano ointment using leaf extractof Justicia adhatoda

Abstract

Background

Nanotechnology is the most expeditiously emerging technology in the field of therapeutics as drug delivery system. Silver has been known to have effective bactericidal properties for centuries. Silver based topical dressing has been widely used as treatment for infection in burns and open wounds.Justicia adhatoda is a full dense evergreen shrub with thick branches, widely distributed in the south Asia and Indo-china. Justicia adhatoda is ethanomedically used as treatment of asthma, cough, bronchitis and tuberculosis, joint pain, lumber pain, sprains, eczema, malaria, rheumatism, swelling, venereal diseases.

Methods

The present research work cost effective and environmental friendly silver nanoparticles were synthesized from the ethanolic extract of Justicia adhatoda as a reducing agent. The characteristic of the obtained silver nanoparticles were studied using UV-Visible spectrophotometry. The bio-synthesized silver nanoparticles infused ointment formulation were evaluated for colour, odour, solubility, PH, melting point, density, spreadability and skin irritation test.

Results

Reduction of silver ions into silver nanoparticles using ethanolic leaf extract of Justicia adhatoda showed colour changed from greyish green to light brown due to excitation of surface vibration in silver nanoparticles.This indicate the formation of silver nanoparticles. The absorption spectrum of the biosynthesized silver nanoparticle showed absorbance peak at 539nm. This indicate that the plasmonic nanoparticles. The physicochemical characteristics of bio-silver nanoparticles infused ointment showed whitecolour, odourless, soluble in petroleum ether, insoluble in water, PH of 4.7, melting point 65^oC, density 39gm/cc, spreadability of 6.12-11.50, no irritation and safe.

Conclusion

The present research draw the conclusion that synthesis of silver nanoparticles from the leaf of Justicia Adhatoda was confirmed by colour change from greyish green to light brown. This indicate the formation of silver nanoparticles. The characterization of the obtained silver nanoparticles were studied using UV-visible spectrophotometer. The bio-silver nanoparticlesinfused ointment physicochemical parameters was successfully evaluated. Further    microbial and clinical tests are warranted to commercialization this product.