Analytical method development and validation for the estimation of  DACLATASVIR in bulk and pharmaceutical dosage form using RP-HPLC

Authors

B. Vijaya
editorijipsr@gmail.com
Assistant professor, Department of Pharmacology, Omega College of Pharmacy, Edulabad, Ghatkesar.
A. Satyaraj Assistant professor, Department of Pharmacology, Omega College of Pharmacy, Edulabad, Ghatkesar.
M. Sailaxmi Assistant professor, Department of Pharmacology, Omega College of Pharmacy, Edulabad, Ghatkesar.

Daclatasvir, RP-HPLC, Reliable, Validation, Assay, Hepatitis, Isocratic

A simple, precise, accurate and linear reverse phase isocratic HPLC was developed and validated for the

determination of Daclatasvir in bulk and tablet dosage forms. Method development was carried out on Zorbax

Eclipse XDB-C18 isocratic column, (250mm × 4.6mm, particle size 5μ, maintained at ambient temperature).

The mobile phase was a mixture of 0.01M Potassium dihydrogen orthophosphate and Acetonitrile (15:85), with

apparent pH of 2.5 and the flow rate was set at 1.0ml/min and UV detection at 284nm. The statistical analysis

shows that the method was found to be accurate, reliable, simple and reproducible. The proposed method was

successfully applied for the quantitative determination of Daclatasvir in bulk form and could be used for routine

analysis with phenomenal accuracy and precisions.

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