Formulation and Evaluation of Herbal Phytosomes with Sinigrin: A Comprehensive Study on Physicochemical Characterization and Stability Analysis

Abstract

Phytosomes represent an advanced delivery system designed to enhance the bioavailability and therapeutic efficacy of herbal compounds. This research article presents a comprehensive investigation into the formulation and evaluation of herbal phytosomes containing sinigrin, a glucosinolate compound with potent pharmacological properties. Three distinct formulations (F1, F2, and F3) were developed using varying ratios of sinigrin, soya-lecithin, and ethanol, utilizing diethyl ether as a solvent and phosphate buffer for optimization. All formulations underwent rigorous physicochemical characterization including pH determination, viscosity analysis, color evaluation, organoleptic assessment (odor and taste), and comprehensive stability analysis under accelerated conditions (40°C ± 2°C, 75% ± 5% RH). The results demonstrated that F2 exhibited optimal physicochemical properties with pH 6.8 ± 0.3, viscosity 45 ± 2 cP, and superior stability with less than 5% drug degradation over 90 days. This study provides valuable insights for the development of herbal phytosomes as effective pharmaceutical formulations.