Reverse phase high performance liquid chromatography method development and validation and validation for estimation of Netlimicin in injection formulation
Keywords:
Netilmicin, Reverse phase, HPLC, Validation, Injection formulation, Symmetry column, C18, Methanol.Abstract
A rapid and precise reverse phase high performance liquid chromatographic method has been developed for the
validated of Netilmicin, in its pure form as well as in injection dosage form. Chromatography was carried out on a
Symmetry C18 (4.6 x 150mm, 5µm) column using a mixture of Methanol and water (45:55% v/v) as the mobile
phase at a flow rate of 0.8ml/min, the detection was carried out at 260nm. The retention time of the Netilmicin was
2.379 ±0.02min respectively. The method produce linear responses in the concentration range of 24-120mg/ml of
Netilmicin. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the
quality control of bulk and pharmaceutical formulations.
Published
Issue
Section
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
