Development and Validation of UV Spectrophotometric Method for Estimation of Alfuzosin Hydrochloride in Bulk and its Tablets

Abstract

This technique is commonly used for the quantitative estimation of the drug from their formulation as well as for studying their metabolites and their estimation in the biological fluids. Alfuzosin is a selective α₁-adrenoreceptor blocking agent. A simple and sensitive UV spectrophotometric method was developed and validated for the determination of alfuzosin hydrochloride in bulk, pharmaceutical formulations. The estimation carried out by using methanol as solvent. The absorbance was measured at 350 nm. The method was linear in the range of 10-30 μg/ml with correlation coefficient value 0.994. The recovery was found to be 97.21-99.86%. The relative standard deviation was found to be less than 2. The method was validated with respect to accuracy, precision, assay, ruggedness, and robustness, limit of detection and limit of quantitation. Due to the flexibility, accuracy and high precision, the developed method can be employed in routine analysis of bulk and dosage forms.