Rp- Hplc Method Development And Validation Of Drotaverin And Mefanamic Acid In Bulk And Pharmaceutical Dosage Forms

Abstract

A rapid and precise reverse phase high performance liquid chromatographic method has been developed for the validated of Thiocolchicoside and Lornoxicam, in its pure form as well as in tablet dosage form. Chromatography was carried out on a Altima C18 (4.6 x 150mm, 5µm) column using a mixture of Methanol and water (5:95% v/v) as the mobile phase at a flow rate of 1.0ml/min, the detection was carried out at 285nm. The retention time of the Thiocolchicoside and Lornoxicam was 2.088, 6.068 ±0.02min respectively. The method produce linear responses in the concentration range of 10-50mg/ml of Thiocolchicoside and 20-100mg/ml of Lornoxicam. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the quality control of bulk and pharmaceutical formulations.