Analytical method development and validation by simultaneous estimation of pioglitazone and teneligliptin in Pharmaceutical dosage form rp-hplc

Abstract

A simple, Accurate, precise method was developed for the simultaneous estimation of the Pioglitazone and Teneligliptin in tablet dosage form. Chromatogram was run through DiscoveyC18 (4.8 x 150mm, 5µm).Mobile phase containing Methanol: 0.01N K2HPo4 taken in the ratio 55:45 was pumped through column at a flow rate of 0.9 ml/min. Buffer used in this method Phosphate buffer and ph is adjusted to 5.4 by adding 0.1% Formic acid. Temperature was maintained at 30°C. Optimized wavelength selected was 240 nm. Retention time of Pioglitazone and Teneligliptin were found to be 2.320 min and 3.256 min. %RSD of the Pioglitazone and Teneligliptin were and found to be 0.6 and 1.5 respectively. %Recovery was obtained as 99.78% and 100.15% for Pioglitazone and Teneligliptin respectively. LOD, LOQ values obtained from regression equations of Pioglitazone and Teneligliptin were 0.02, 0.07 and 0.07, 0.21 respectively. Regression equation of Pioglitazone is y = 27728x + 4960.5. And y = 38863x + 7130.7 of Teneligliptin . Retention times were decreased and that run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries