Newer RP-HPLC Method Development And Validation For The Simultaneous Estimation Of Diloxanide Furoate, Tinidazole In Dosage Form

Abstract

A rapid and precise reverse phase high performance liquid chromatographic method has been developed for the validated of Diloxanide and Tinidazole, in its pure form as well as in tablet dosage form. Chromatography was carried out on a Phenomenex Gemini C18 (4.6 x 150mm, 5µm) column using a mixture of Methanol: Water (25:75% v/v) as the mobile phase at a flow rate of 1.0ml/min, the detection was carried out at 240 nm. The retention time of the Tinidazole and Diloxanide was 2.256, 5.427 ±0.02min respectively. The method produce linear responses in the concentration range of 5-25mg/ml of Tinidazole and 25-125mg/ml of Diloxanide. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the quality control of bulk and pharmaceutical formulations.