SIMULTANEOUS ESTIMATION AND VALIDATION OF ATENOLOL, HYDROCHLORO THIAZIDE AND LOSARTAN K IN TABLET DOSAGE FORM BY RP-HPLC METHOD.

Abstract

A simple RP-HPLC method was developed and validated for simultaneous estimation of Atenolol, Hydrochloro thiazide and Losartan K from pharmaceutical dosage forms. A sensitive chromatographic separation was accomplished on thermo scientific C18 column (20 cm×4.6 mm, 5µ) with mobile phase consisting of Acetonitrile : phosphate buffer (pH 3.6 adjusted with anhydrous disodium hydrogen phosphate) in the ratio of 70:30 v/v, at a flow rate of 1.2 ml/min and eluents monitored at 229 nm. The developed method was validated in terms of accuracy, precision, linearity and limit of detection, limit of quantification, robustness and solution stability. The proposed method can be used for the estimation of these drugs in combined pharmaceutical dosage forms.