New analytical method development and validation for the simultaneous  estimation of tranexamic acid and mefenamic acid in pharmaceutical dosage  forms

Authors

Devikasubramaniyan.G
editorijipsr@gmail.com
Department of Pharmaceutical Analysis and Quality Assurance, Bomma Institute of pharmacy, Affiliated to JNTUH, Allipuram, Khammam-507318. Telangana, India.
Rameshpetchi Rajendran Department of Pharmaceutical Analysis and Quality Assurance, Bomma Institute of pharmacy, Affiliated to JNTUH, Allipuram, Khammam-507318. Telangana, India.
Marasakatla Aruna Department of Pharmaceutical Analysis and Quality Assurance, Bomma Institute of pharmacy, Affiliated to JNTUH, Allipuram, Khammam-507318. Telangana, India.

Tranexamic acid Mefenamic acid, Simultaneous estimation, RP-HPLC method.

A selective and sensitive reverse phase high performance liquid chromatography (RP-HPLC) has been developed for

the separation and quantification of Tranexamic acid and Mefenamic acid in tablet dosage form and validated .The

determination was carried out using Thermosil C18 column (25 cm ×4.6 mm id) as a stationary phase and mobile

phase comprised of Methanol: Sodium acetate buffer in proportion of 65:35(v/v) with pH adjusted to 3±0.5 by using

orthophosphoricacid. The flow rate was 1.0ml/min and the eluent was monitored at 256nm.The retention time of

Tranexamic acid and Mefenamic acid were 2.45 ±0.028 min and 4.31±0.018 min respectively.. The Coefficient of

correlation and percentage recoveries of Tranexamic acid and Mefenamic acid were 0.9986 and 100.0l % and 0.9994

and 99.98% respectively. The method is validated for accuracy, Precision, ruggedness and Robustness. The proposed

method is successfully applied for the simultaneous determination of both drugs in commercial tablet preparation.

The results of the analysis have been validated statistically and by recovery studies.

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