Analytical method development for the estimation of everolimus by rp-hplc

Abstract

In reversed phase systems the strong attractive forces between water molecules arising from the 3-dimentional inter molecular hydrogen bonded network, from a structure of water that must be distorted or disrupted when a solute is dissolved. Only higher polar or ionic solutes can interact with the water structure. Non- polar solutes are squeezed out of the mobile phase and are relatively insoluble in it but with the hydrocarbon moieties of the stationary phase.

A sensitive & selective RP-HPLC method has been developed & validated for the analysis of Everolimus API. Further the proposed RP-HPLC method has excellent sensitivity, precision and reproducibility.