Production of Pectin Lyase by fungi isolated from agro waste

Abstract

In the present investigation totally 25 fungal species alone were isolated during the study period from three different samples

such as dead organic, fruit and vegetable soil samples. The fungal cultures were identified by standard manual and kept on PDA

medium.The present data demonstrate that maximum enzyme activity was exhibitedin A.niger (5.2 IU/ml)and P.citrinum (4.8

IU/ml)in both plate assay and quantification assay. The minimum enzyme activity was expressed as Curvularia lunata,

Rhizoctonia solani; Aspergillus flavus, A.lunchensis and A.nidulans. Pectin lyase production was increased by optimizing

various fermentation parameters. The enzyme activity was assayed over a pH range of 5.0 to 9.0. The enzyme activity was

assayed at different temperature (15-35°C).This was agreed with present findings i.e., 6.1 IU/ml of pectin lyase activity was

recorded after 9 days of incubation by A.niger and P.citrinum was 6.9 IU/ml maximum amount of protein was also recorded

after 9 days of incubation.In the present study, partial purification of Pectin lyase enzyme fromA.niger and P.citrinum was

carried out by dialysis followed by Ammonium sulphate precipitation method.