Method development and validation for the simultaneous estimation of lamivudine and zidovudine in pure form and marketed pharmaceutical dosage form by using RP-HPLC

Abstract

A rapid and precise reverse phase high performance liquid chromatographic method has been developed for the

validated of Lamivudine and Zidovudine, in its pure form as well as in tablet dosage form. Chromatography

was carried out on a Altima C18 (4.6 x 150mm, 5µm) column using a mixture of Phosphate buffer pH4.6,

Methanol and ACN (65:25:10 v/v) as the mobile phase at a flow rate of 1.0ml/min, the de tection was carried

out at 265nm. The retention time of the Lamivudine and Zidovudine was 2.088, 6.068 ±0.02min respectively.

The method produce linear responses in the concentration range of 10-50mg/ml of Lamivudine and 20-

100mg/ml of Zidovudine. The method precision for the determination of assay was below 2.0%RSD. The

method is useful in the quality control of bulk and pharmaceutical formulations.