Screening of industrial important enzymes from microfungal isolates of agricultural soil and litter samples from Sivakasi, Tamil Nadu

Authors

  • Muthukrishnan S Department of Biotechnology, Ayya Nadar Janaki Ammal College, Sivakasi-626124, Tamil Nadu, India.
  • Rajaram V Department of Biotechnology, Ayya Nadar Janaki Ammal College, Sivakasi-626124, Tamil Nadu, India.

Keywords:

Micro-fungal diversity, Fungal growth, Industrial enzymes, Plate assay.

Abstract

Fungi colonization and subsequent decomposition of plant substrate depended as much on the ability of the

fungi to produce enzymes necessary to degrade particular plant polymers. The degradation of the substrate is

achieved only through a range of enzymes produced by the fungi. In recent year importance is given to our

understanding of the diversity of fungi and has great scope for industrial application. Seven micro -fungal

species were isolated from three samples such as agricultural soil, leaf litter soil, and wood debris soil. Fungal

species were isolated through pure culture techniques and their pH tolerant studies between 6 and 8. The

growth characteristic was studied in relation to alkalinity and pH. The fungal species were tested for amylase,

protease, cellulose, lipase enzyme through plate assays. The maximum fungal enzyme activities were screened

in Aspergillus flavus for amylase, cellulase for Aspergillus niger, protease for Aspergillus ochraceus, lipase for

Aspergillus terreus.

Dimensions

Published

2020-01-15