Screening of industrial important enzymes from microfungal isolates of agricultural soil and litter samples from Sivakasi, Tamil Nadu
Keywords:
Micro-fungal diversity, Fungal growth, Industrial enzymes, Plate assay.Abstract
Fungi colonization and subsequent decomposition of plant substrate depended as much on the ability of the
fungi to produce enzymes necessary to degrade particular plant polymers. The degradation of the substrate is
achieved only through a range of enzymes produced by the fungi. In recent year importance is given to our
understanding of the diversity of fungi and has great scope for industrial application. Seven micro -fungal
species were isolated from three samples such as agricultural soil, leaf litter soil, and wood debris soil. Fungal
species were isolated through pure culture techniques and their pH tolerant studies between 6 and 8. The
growth characteristic was studied in relation to alkalinity and pH. The fungal species were tested for amylase,
protease, cellulose, lipase enzyme through plate assays. The maximum fungal enzyme activities were screened
in Aspergillus flavus for amylase, cellulase for Aspergillus niger, protease for Aspergillus ochraceus, lipase for
Aspergillus terreus.
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