SIMULTANEOUS HPTLC ESTIMATION OF NAPROXEN AND PANTOPRAZOLE FROM CAPSULE DOSAGE FORMS.

Abstract

The aim of the present study was to develop simple, accurate and precise method for simultaneous

determination of Naproxen and Pantoprazole in capsules by HPTLC method. The proposed method shows that

the chromatographic layer gives the best separation of the two component in the mobile phase consisting of

Toluene: Methanol: Formic acid (8.5:1.3:0.2); other system like Chloroform: Ethyl acetate: Methanol: water

(5.3:2.7:1.5:0.5); where the components move along with solvent front; chloroform: Methanol: Formic acid

(10:2.5:0.5) where the component (Pantoprazole) not moved. Finally Toluene: Chloroform: Methanol: Formic

acid (3.5:2.1:4.2:0.2) gave the complete separation with Rf values of Naproxen and Pantoprazole were 0.41 ±

0.02 and 0.51 ± 0.02 respectively. The method was validated with respect to accuracy, precision, limit of

detection and limit of quantification. From the results it can be concluded that new HPTLC method could be

used in routine analysis for simultaneous determination of Naproxen and Pantoprazole in combined dosage

forms.